positive control histone h3 antibody (Cell Signaling Technology Inc)
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Positive Control Histone H3 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 4296 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 97 stars, based on 4296 article reviews
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Negative Control:Article Title: Characterization of GJB2 cis-regulatory elements in the DFNB1 locus. Article Snippet: Although most disease-causing variants are within coding region of genes, it is now well established that cis-acting regulatory sequences, depending on 3D-chromatin organization, are required for temporal and spatial control of gene expression.. Disruptions of such regulatory elements and/or chromatin conformation are likely to play a critical role in human genetic disease.. Hence, recurrent monoallelic cases, who present the most common hereditary type of nonsyndromic hearing loss (i.e., DFNB1), carry only one identified pathogenic allele. Article Title: CFTR Cooperative Cis -Regulatory Elements in Intestinal Cells Article Snippet: The Adaptive Focused AcousticsTM (AFA) Technology from Covaris was used in addition to enzymatic digestion with micrococcal nuclease (0.5 μL) in order to produce DNA fragments of 150 to 900 bp. .. Chromatin was precleared with protein G magnetic beads (Cell Signaling Technology) for 2 h at 4 °C and immunoprecipitation with HNF1α (#89670, Cell Signaling Technology), p300 (#54062, Cell Signaling Technology, Danvers, MA, USA), CDX2 (#12306, Cell Signaling Technology), TCF4 (#2569, Cell Signaling Technology), FOXA1 (#53528, Cell Signaling Technology), and FOXA2 (#8186, Cell Signaling Technology) specific antibodies, a negative control IgG antibody or a Article Title: Hepatocyte SLCO4C1 is a cAMP uptake transporter for inhibiting lipogenesis and a therapeutic target for MASLD. Article Snippet: Next, 1μl of Micrococcal Nuclease (CST, Boston, USA, Cat:10011) per IP sample was used to digest chromatin into varying lengths of fragments. .. Subsequently, the digested chromatin fragments were probed with 1μg anti-EGR1 antibody (CST, Boston, USA, Cat:4153S), Article Title: 3D Chromatin Organization Involving MEIS1 Factor in the cis -Regulatory Landscape of GJB2 Article Snippet: The Adaptive Focused AcousticsTM (AFA) Technology from Covaris (Waltham, MA, USA) was used to sonicate chromatin for 4 min with 5% of sonication (75 Watts) two times. .. Chromatin was precleared with protein G agarose beads (Cell Signaling Technology, Waltham, MA, USA) for 2 h at 4 °C and immunoprecipitated with 8.5 μL of MEIS1-specific antibodies (Atlas Antibodies HPA056000), a negative control IgG antibody, or a Article Title: Analysis of long-range interactions in primary human cells identifies cooperative CFTR regulatory elements Article Snippet: The Adaptive Focused AcousticsTM (AFA) Technology from Covaris was used in addition to enzymatic digestion with microccocal nuclease in order to produce DNA fragments of 150 to 900 bp. .. Chromatin was precleared with protein G magnetic beads (Cell Signaling Technology) for 2 h at 4°C and immunoprecipitation with the CTCF specific antibodies, a negative control IgG antibody or a Article Title: Transcriptional co-factor Transducin beta-like (TBL) 1 acts as a checkpoint in pancreatic cancer malignancy Article Snippet: .. Antibodies were added as follows and incubated over night at 4°C: 1 μg negative control Normal Rabbit IgG (#2729, Cell Signaling), 10 μl Article Title: Cx32 mediates norepinephrine-promoted EGFR-TKI resistance in a gap junction-independent manner in non-small-cell lung cancer. Article Snippet: Funding information National Natural Science Foundation of China, Grant/Award Number: 81473234; 81373439; Joint Fund of the National Natural Science Foundation of China, Grant/Award Number: U1303221; construction of technique plate for evaluation of the pharmacodynamics of new drugs in Xinjiang from the Department of Science and Technology of Guangdong province, Grant/Award Number: 2014A020209032; Department of Science and Technology of Guangdong Province, Grant/Award Number: 20160908; Fundamental Research Funds for the Central Universities, Grant/Award Number: 16ykjc01 Abstract The second‐generation EGFR‐TKI Afatinib is an irreversible ErbB family blocker used to treat patients with non‐small‐cell lung cancer (NSCLC).. Unfortunately, resistance to this drug develops over time, and patients are always under great psychological pressure.. A previous study showed that chronic stress hormones participate in EGFR‐TKI resistance via β2‐AR signaling via an IL‐6 dependent mechanism. Positive Control:Article Title: Characterization of GJB2 cis-regulatory elements in the DFNB1 locus. Article Snippet: Although most disease-causing variants are within coding region of genes, it is now well established that cis-acting regulatory sequences, depending on 3D-chromatin organization, are required for temporal and spatial control of gene expression.. Disruptions of such regulatory elements and/or chromatin conformation are likely to play a critical role in human genetic disease.. Hence, recurrent monoallelic cases, who present the most common hereditary type of nonsyndromic hearing loss (i.e., DFNB1), carry only one identified pathogenic allele. Article Title: CFTR Cooperative Cis -Regulatory Elements in Intestinal Cells Article Snippet: The Adaptive Focused AcousticsTM (AFA) Technology from Covaris was used in addition to enzymatic digestion with micrococcal nuclease (0.5 μL) in order to produce DNA fragments of 150 to 900 bp. .. Chromatin was precleared with protein G magnetic beads (Cell Signaling Technology) for 2 h at 4 °C and immunoprecipitation with HNF1α (#89670, Cell Signaling Technology), p300 (#54062, Cell Signaling Technology, Danvers, MA, USA), CDX2 (#12306, Cell Signaling Technology), TCF4 (#2569, Cell Signaling Technology), FOXA1 (#53528, Cell Signaling Technology), and FOXA2 (#8186, Cell Signaling Technology) specific antibodies, a negative control IgG antibody or a Article Title: Hepatocyte SLCO4C1 is a cAMP uptake transporter for inhibiting lipogenesis and a therapeutic target for MASLD. Article Snippet: Next, 1μl of Micrococcal Nuclease (CST, Boston, USA, Cat:10011) per IP sample was used to digest chromatin into varying lengths of fragments. .. Subsequently, the digested chromatin fragments were probed with 1μg anti-EGR1 antibody (CST, Boston, USA, Cat:4153S), Article Title: 3D Chromatin Organization Involving MEIS1 Factor in the cis -Regulatory Landscape of GJB2 Article Snippet: The Adaptive Focused AcousticsTM (AFA) Technology from Covaris (Waltham, MA, USA) was used to sonicate chromatin for 4 min with 5% of sonication (75 Watts) two times. .. Chromatin was precleared with protein G agarose beads (Cell Signaling Technology, Waltham, MA, USA) for 2 h at 4 °C and immunoprecipitated with 8.5 μL of MEIS1-specific antibodies (Atlas Antibodies HPA056000), a negative control IgG antibody, or a Article Title: Analysis of long-range interactions in primary human cells identifies cooperative CFTR regulatory elements Article Snippet: The Adaptive Focused AcousticsTM (AFA) Technology from Covaris was used in addition to enzymatic digestion with microccocal nuclease in order to produce DNA fragments of 150 to 900 bp. .. Chromatin was precleared with protein G magnetic beads (Cell Signaling Technology) for 2 h at 4°C and immunoprecipitation with the CTCF specific antibodies, a negative control IgG antibody or a Article Title: Transcriptional co-factor Transducin beta-like (TBL) 1 acts as a checkpoint in pancreatic cancer malignancy Article Snippet: .. Antibodies were added as follows and incubated over night at 4°C: 1 μg negative control Normal Rabbit IgG (#2729, Cell Signaling), 10 μl Article Title: Cx32 mediates norepinephrine-promoted EGFR-TKI resistance in a gap junction-independent manner in non-small-cell lung cancer. Article Snippet: Funding information National Natural Science Foundation of China, Grant/Award Number: 81473234; 81373439; Joint Fund of the National Natural Science Foundation of China, Grant/Award Number: U1303221; construction of technique plate for evaluation of the pharmacodynamics of new drugs in Xinjiang from the Department of Science and Technology of Guangdong province, Grant/Award Number: 2014A020209032; Department of Science and Technology of Guangdong Province, Grant/Award Number: 20160908; Fundamental Research Funds for the Central Universities, Grant/Award Number: 16ykjc01 Abstract The second‐generation EGFR‐TKI Afatinib is an irreversible ErbB family blocker used to treat patients with non‐small‐cell lung cancer (NSCLC).. Unfortunately, resistance to this drug develops over time, and patients are always under great psychological pressure.. A previous study showed that chronic stress hormones participate in EGFR‐TKI resistance via β2‐AR signaling via an IL‐6 dependent mechanism. Magnetic Beads:Article Title: CFTR Cooperative Cis -Regulatory Elements in Intestinal Cells Article Snippet: The Adaptive Focused AcousticsTM (AFA) Technology from Covaris was used in addition to enzymatic digestion with micrococcal nuclease (0.5 μL) in order to produce DNA fragments of 150 to 900 bp. .. Chromatin was precleared with protein G magnetic beads (Cell Signaling Technology) for 2 h at 4 °C and immunoprecipitation with HNF1α (#89670, Cell Signaling Technology), p300 (#54062, Cell Signaling Technology, Danvers, MA, USA), CDX2 (#12306, Cell Signaling Technology), TCF4 (#2569, Cell Signaling Technology), FOXA1 (#53528, Cell Signaling Technology), and FOXA2 (#8186, Cell Signaling Technology) specific antibodies, a negative control IgG antibody or a Article Title: Analysis of long-range interactions in primary human cells identifies cooperative CFTR regulatory elements Article Snippet: The Adaptive Focused AcousticsTM (AFA) Technology from Covaris was used in addition to enzymatic digestion with microccocal nuclease in order to produce DNA fragments of 150 to 900 bp. .. Chromatin was precleared with protein G magnetic beads (Cell Signaling Technology) for 2 h at 4°C and immunoprecipitation with the CTCF specific antibodies, a negative control IgG antibody or a Immunoprecipitation:Article Title: CFTR Cooperative Cis -Regulatory Elements in Intestinal Cells Article Snippet: The Adaptive Focused AcousticsTM (AFA) Technology from Covaris was used in addition to enzymatic digestion with micrococcal nuclease (0.5 μL) in order to produce DNA fragments of 150 to 900 bp. .. Chromatin was precleared with protein G magnetic beads (Cell Signaling Technology) for 2 h at 4 °C and immunoprecipitation with HNF1α (#89670, Cell Signaling Technology), p300 (#54062, Cell Signaling Technology, Danvers, MA, USA), CDX2 (#12306, Cell Signaling Technology), TCF4 (#2569, Cell Signaling Technology), FOXA1 (#53528, Cell Signaling Technology), and FOXA2 (#8186, Cell Signaling Technology) specific antibodies, a negative control IgG antibody or a Article Title: 3D Chromatin Organization Involving MEIS1 Factor in the cis -Regulatory Landscape of GJB2 Article Snippet: The Adaptive Focused AcousticsTM (AFA) Technology from Covaris (Waltham, MA, USA) was used to sonicate chromatin for 4 min with 5% of sonication (75 Watts) two times. .. Chromatin was precleared with protein G agarose beads (Cell Signaling Technology, Waltham, MA, USA) for 2 h at 4 °C and immunoprecipitated with 8.5 μL of MEIS1-specific antibodies (Atlas Antibodies HPA056000), a negative control IgG antibody, or a Article Title: Analysis of long-range interactions in primary human cells identifies cooperative CFTR regulatory elements Article Snippet: The Adaptive Focused AcousticsTM (AFA) Technology from Covaris was used in addition to enzymatic digestion with microccocal nuclease in order to produce DNA fragments of 150 to 900 bp. .. Chromatin was precleared with protein G magnetic beads (Cell Signaling Technology) for 2 h at 4°C and immunoprecipitation with the CTCF specific antibodies, a negative control IgG antibody or a Article Title: Cx32 mediates norepinephrine-promoted EGFR-TKI resistance in a gap junction-independent manner in non-small-cell lung cancer. Article Snippet: Funding information National Natural Science Foundation of China, Grant/Award Number: 81473234; 81373439; Joint Fund of the National Natural Science Foundation of China, Grant/Award Number: U1303221; construction of technique plate for evaluation of the pharmacodynamics of new drugs in Xinjiang from the Department of Science and Technology of Guangdong province, Grant/Award Number: 2014A020209032; Department of Science and Technology of Guangdong Province, Grant/Award Number: 20160908; Fundamental Research Funds for the Central Universities, Grant/Award Number: 16ykjc01 Abstract The second‐generation EGFR‐TKI Afatinib is an irreversible ErbB family blocker used to treat patients with non‐small‐cell lung cancer (NSCLC).. Unfortunately, resistance to this drug develops over time, and patients are always under great psychological pressure.. A previous study showed that chronic stress hormones participate in EGFR‐TKI resistance via β2‐AR signaling via an IL‐6 dependent mechanism. Incubation:Article Title: Hepatocyte SLCO4C1 is a cAMP uptake transporter for inhibiting lipogenesis and a therapeutic target for MASLD. Article Snippet: Next, 1μl of Micrococcal Nuclease (CST, Boston, USA, Cat:10011) per IP sample was used to digest chromatin into varying lengths of fragments. .. Subsequently, the digested chromatin fragments were probed with 1μg anti-EGR1 antibody (CST, Boston, USA, Cat:4153S), Article Title: Transcriptional co-factor Transducin beta-like (TBL) 1 acts as a checkpoint in pancreatic cancer malignancy Article Snippet: .. Antibodies were added as follows and incubated over night at 4°C: 1 μg negative control Normal Rabbit IgG (#2729, Cell Signaling), 10 μl Polymerase Chain Reaction:Article Title: Hepatocyte SLCO4C1 is a cAMP uptake transporter for inhibiting lipogenesis and a therapeutic target for MASLD. Article Snippet: Next, 1μl of Micrococcal Nuclease (CST, Boston, USA, Cat:10011) per IP sample was used to digest chromatin into varying lengths of fragments. .. Subsequently, the digested chromatin fragments were probed with 1μg anti-EGR1 antibody (CST, Boston, USA, Cat:4153S), Amplification:Article Title: Hepatocyte SLCO4C1 is a cAMP uptake transporter for inhibiting lipogenesis and a therapeutic target for MASLD. Article Snippet: Next, 1μl of Micrococcal Nuclease (CST, Boston, USA, Cat:10011) per IP sample was used to digest chromatin into varying lengths of fragments. .. Subsequently, the digested chromatin fragments were probed with 1μg anti-EGR1 antibody (CST, Boston, USA, Cat:4153S), |
