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positive control histone h3 antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc positive control histone h3 antibody
    Positive Control Histone H3 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 4296 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/positive+control+histone+h3+antibody/Histone+H3+Antibody/pm41826328-281-15-20
    Average 97 stars, based on 4296 article reviews
    positive control histone h3 antibody - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Negative Control:

    Article Title: Characterization of GJB2 cis-regulatory elements in the DFNB1 locus.
    Article Snippet: Although most disease-causing variants are within coding region of genes, it is now well established that cis-acting regulatory sequences, depending on 3D-chromatin organization, are required for temporal and spatial control of gene expression.. Disruptions of such regulatory elements and/or chromatin conformation are likely to play a critical role in human genetic disease.. Hence, recurrent monoallelic cases, who present the most common hereditary type of nonsyndromic hearing loss (i.e., DFNB1), carry only one identified pathogenic allele.

    Article Title: CFTR Cooperative Cis -Regulatory Elements in Intestinal Cells
    Article Snippet: The Adaptive Focused AcousticsTM (AFA) Technology from Covaris was used in addition to enzymatic digestion with micrococcal nuclease (0.5 μL) in order to produce DNA fragments of 150 to 900 bp. .. Chromatin was precleared with protein G magnetic beads (Cell Signaling Technology) for 2 h at 4 °C and immunoprecipitation with HNF1α (#89670, Cell Signaling Technology), p300 (#54062, Cell Signaling Technology, Danvers, MA, USA), CDX2 (#12306, Cell Signaling Technology), TCF4 (#2569, Cell Signaling Technology), FOXA1 (#53528, Cell Signaling Technology), and FOXA2 (#8186, Cell Signaling Technology) specific antibodies, a negative control IgG antibody or a positive control Histone H3 antibody (Cell Signaling Technology) were carried out overnight at 4 °C. ..

    Article Title: Hepatocyte SLCO4C1 is a cAMP uptake transporter for inhibiting lipogenesis and a therapeutic target for MASLD.
    Article Snippet: Next, 1μl of Micrococcal Nuclease (CST, Boston, USA, Cat:10011) per IP sample was used to digest chromatin into varying lengths of fragments. .. Subsequently, the digested chromatin fragments were probed with 1μg anti-EGR1 antibody (CST, Boston, USA, Cat:4153S), positive control Histone H3 antibody (CST, Boston, USA, Cat:4620) or negative control Normal Rabbit IgG (CST, Boston, USA, Cat:2729) and incubated at 4°C for 12 h. The resulting immunoprecipitants containing specific DNA were subjected to PCR amplification using specific primers (Supplementary Table3). .. Slco4c1 overexpression by adeno-associated virus 8 (AAV8) The liver-specific AAV8 virions for the overexpression of Slco4c1-TBG-luciferase or controlTBG-luciferase were constructed and generated by BrainVTA (Wuhan, China).

    Article Title: 3D Chromatin Organization Involving MEIS1 Factor in the cis -Regulatory Landscape of GJB2
    Article Snippet: The Adaptive Focused AcousticsTM (AFA) Technology from Covaris (Waltham, MA, USA) was used to sonicate chromatin for 4 min with 5% of sonication (75 Watts) two times. .. Chromatin was precleared with protein G agarose beads (Cell Signaling Technology, Waltham, MA, USA) for 2 h at 4 °C and immunoprecipitated with 8.5 μL of MEIS1-specific antibodies (Atlas Antibodies HPA056000), a negative control IgG antibody, or a positive control Histone H3 antibody (Cell Signaling Technology) overnight at 4 °C. .. PCRs were performed in triplicate using the HotStarTaq Master Mix (QIAGEN), and the primer sequences used are listed in .

    Article Title: Analysis of long-range interactions in primary human cells identifies cooperative CFTR regulatory elements
    Article Snippet: The Adaptive Focused AcousticsTM (AFA) Technology from Covaris was used in addition to enzymatic digestion with microccocal nuclease in order to produce DNA fragments of 150 to 900 bp. .. Chromatin was precleared with protein G magnetic beads (Cell Signaling Technology) for 2 h at 4°C and immunoprecipitation with the CTCF specific antibodies, a negative control IgG antibody or a positive control Histone H3 antibody (Cell Signaling Technology) were carried out overnight at 4°C. ..

    Article Title: Transcriptional co-factor Transducin beta-like (TBL) 1 acts as a checkpoint in pancreatic cancer malignancy
    Article Snippet: .. Antibodies were added as follows and incubated over night at 4°C: 1 μg negative control Normal Rabbit IgG (#2729, Cell Signaling), 10 μl positive control Histone H3 antibody (#4620, Cell Signaling), and 10 μl TBL1X-antibody (ab24548, Abcam). ..

    Article Title: Cx32 mediates norepinephrine-promoted EGFR-TKI resistance in a gap junction-independent manner in non-small-cell lung cancer.
    Article Snippet: Funding information National Natural Science Foundation of China, Grant/Award Number: 81473234; 81373439; Joint Fund of the National Natural Science Foundation of China, Grant/Award Number: U1303221; construction of technique plate for evaluation of the pharmacodynamics of new drugs in Xinjiang from the Department of Science and Technology of Guangdong province, Grant/Award Number: 2014A020209032; Department of Science and Technology of Guangdong Province, Grant/Award Number: 20160908; Fundamental Research Funds for the Central Universities, Grant/Award Number: 16ykjc01 Abstract The second‐generation EGFR‐TKI Afatinib is an irreversible ErbB family blocker used to treat patients with non‐small‐cell lung cancer (NSCLC).. Unfortunately, resistance to this drug develops over time, and patients are always under great psychological pressure.. A previous study showed that chronic stress hormones participate in EGFR‐TKI resistance via β2‐AR signaling via an IL‐6 dependent mechanism.

    Positive Control:

    Article Title: Characterization of GJB2 cis-regulatory elements in the DFNB1 locus.
    Article Snippet: Although most disease-causing variants are within coding region of genes, it is now well established that cis-acting regulatory sequences, depending on 3D-chromatin organization, are required for temporal and spatial control of gene expression.. Disruptions of such regulatory elements and/or chromatin conformation are likely to play a critical role in human genetic disease.. Hence, recurrent monoallelic cases, who present the most common hereditary type of nonsyndromic hearing loss (i.e., DFNB1), carry only one identified pathogenic allele.

    Article Title: CFTR Cooperative Cis -Regulatory Elements in Intestinal Cells
    Article Snippet: The Adaptive Focused AcousticsTM (AFA) Technology from Covaris was used in addition to enzymatic digestion with micrococcal nuclease (0.5 μL) in order to produce DNA fragments of 150 to 900 bp. .. Chromatin was precleared with protein G magnetic beads (Cell Signaling Technology) for 2 h at 4 °C and immunoprecipitation with HNF1α (#89670, Cell Signaling Technology), p300 (#54062, Cell Signaling Technology, Danvers, MA, USA), CDX2 (#12306, Cell Signaling Technology), TCF4 (#2569, Cell Signaling Technology), FOXA1 (#53528, Cell Signaling Technology), and FOXA2 (#8186, Cell Signaling Technology) specific antibodies, a negative control IgG antibody or a positive control Histone H3 antibody (Cell Signaling Technology) were carried out overnight at 4 °C. ..

    Article Title: Hepatocyte SLCO4C1 is a cAMP uptake transporter for inhibiting lipogenesis and a therapeutic target for MASLD.
    Article Snippet: Next, 1μl of Micrococcal Nuclease (CST, Boston, USA, Cat:10011) per IP sample was used to digest chromatin into varying lengths of fragments. .. Subsequently, the digested chromatin fragments were probed with 1μg anti-EGR1 antibody (CST, Boston, USA, Cat:4153S), positive control Histone H3 antibody (CST, Boston, USA, Cat:4620) or negative control Normal Rabbit IgG (CST, Boston, USA, Cat:2729) and incubated at 4°C for 12 h. The resulting immunoprecipitants containing specific DNA were subjected to PCR amplification using specific primers (Supplementary Table3). .. Slco4c1 overexpression by adeno-associated virus 8 (AAV8) The liver-specific AAV8 virions for the overexpression of Slco4c1-TBG-luciferase or controlTBG-luciferase were constructed and generated by BrainVTA (Wuhan, China).

    Article Title: 3D Chromatin Organization Involving MEIS1 Factor in the cis -Regulatory Landscape of GJB2
    Article Snippet: The Adaptive Focused AcousticsTM (AFA) Technology from Covaris (Waltham, MA, USA) was used to sonicate chromatin for 4 min with 5% of sonication (75 Watts) two times. .. Chromatin was precleared with protein G agarose beads (Cell Signaling Technology, Waltham, MA, USA) for 2 h at 4 °C and immunoprecipitated with 8.5 μL of MEIS1-specific antibodies (Atlas Antibodies HPA056000), a negative control IgG antibody, or a positive control Histone H3 antibody (Cell Signaling Technology) overnight at 4 °C. .. PCRs were performed in triplicate using the HotStarTaq Master Mix (QIAGEN), and the primer sequences used are listed in .

    Article Title: Analysis of long-range interactions in primary human cells identifies cooperative CFTR regulatory elements
    Article Snippet: The Adaptive Focused AcousticsTM (AFA) Technology from Covaris was used in addition to enzymatic digestion with microccocal nuclease in order to produce DNA fragments of 150 to 900 bp. .. Chromatin was precleared with protein G magnetic beads (Cell Signaling Technology) for 2 h at 4°C and immunoprecipitation with the CTCF specific antibodies, a negative control IgG antibody or a positive control Histone H3 antibody (Cell Signaling Technology) were carried out overnight at 4°C. ..

    Article Title: Transcriptional co-factor Transducin beta-like (TBL) 1 acts as a checkpoint in pancreatic cancer malignancy
    Article Snippet: .. Antibodies were added as follows and incubated over night at 4°C: 1 μg negative control Normal Rabbit IgG (#2729, Cell Signaling), 10 μl positive control Histone H3 antibody (#4620, Cell Signaling), and 10 μl TBL1X-antibody (ab24548, Abcam). ..

    Article Title: Cx32 mediates norepinephrine-promoted EGFR-TKI resistance in a gap junction-independent manner in non-small-cell lung cancer.
    Article Snippet: Funding information National Natural Science Foundation of China, Grant/Award Number: 81473234; 81373439; Joint Fund of the National Natural Science Foundation of China, Grant/Award Number: U1303221; construction of technique plate for evaluation of the pharmacodynamics of new drugs in Xinjiang from the Department of Science and Technology of Guangdong province, Grant/Award Number: 2014A020209032; Department of Science and Technology of Guangdong Province, Grant/Award Number: 20160908; Fundamental Research Funds for the Central Universities, Grant/Award Number: 16ykjc01 Abstract The second‐generation EGFR‐TKI Afatinib is an irreversible ErbB family blocker used to treat patients with non‐small‐cell lung cancer (NSCLC).. Unfortunately, resistance to this drug develops over time, and patients are always under great psychological pressure.. A previous study showed that chronic stress hormones participate in EGFR‐TKI resistance via β2‐AR signaling via an IL‐6 dependent mechanism.

    Magnetic Beads:

    Article Title: CFTR Cooperative Cis -Regulatory Elements in Intestinal Cells
    Article Snippet: The Adaptive Focused AcousticsTM (AFA) Technology from Covaris was used in addition to enzymatic digestion with micrococcal nuclease (0.5 μL) in order to produce DNA fragments of 150 to 900 bp. .. Chromatin was precleared with protein G magnetic beads (Cell Signaling Technology) for 2 h at 4 °C and immunoprecipitation with HNF1α (#89670, Cell Signaling Technology), p300 (#54062, Cell Signaling Technology, Danvers, MA, USA), CDX2 (#12306, Cell Signaling Technology), TCF4 (#2569, Cell Signaling Technology), FOXA1 (#53528, Cell Signaling Technology), and FOXA2 (#8186, Cell Signaling Technology) specific antibodies, a negative control IgG antibody or a positive control Histone H3 antibody (Cell Signaling Technology) were carried out overnight at 4 °C. ..

    Article Title: Analysis of long-range interactions in primary human cells identifies cooperative CFTR regulatory elements
    Article Snippet: The Adaptive Focused AcousticsTM (AFA) Technology from Covaris was used in addition to enzymatic digestion with microccocal nuclease in order to produce DNA fragments of 150 to 900 bp. .. Chromatin was precleared with protein G magnetic beads (Cell Signaling Technology) for 2 h at 4°C and immunoprecipitation with the CTCF specific antibodies, a negative control IgG antibody or a positive control Histone H3 antibody (Cell Signaling Technology) were carried out overnight at 4°C. ..

    Immunoprecipitation:

    Article Title: CFTR Cooperative Cis -Regulatory Elements in Intestinal Cells
    Article Snippet: The Adaptive Focused AcousticsTM (AFA) Technology from Covaris was used in addition to enzymatic digestion with micrococcal nuclease (0.5 μL) in order to produce DNA fragments of 150 to 900 bp. .. Chromatin was precleared with protein G magnetic beads (Cell Signaling Technology) for 2 h at 4 °C and immunoprecipitation with HNF1α (#89670, Cell Signaling Technology), p300 (#54062, Cell Signaling Technology, Danvers, MA, USA), CDX2 (#12306, Cell Signaling Technology), TCF4 (#2569, Cell Signaling Technology), FOXA1 (#53528, Cell Signaling Technology), and FOXA2 (#8186, Cell Signaling Technology) specific antibodies, a negative control IgG antibody or a positive control Histone H3 antibody (Cell Signaling Technology) were carried out overnight at 4 °C. ..

    Article Title: 3D Chromatin Organization Involving MEIS1 Factor in the cis -Regulatory Landscape of GJB2
    Article Snippet: The Adaptive Focused AcousticsTM (AFA) Technology from Covaris (Waltham, MA, USA) was used to sonicate chromatin for 4 min with 5% of sonication (75 Watts) two times. .. Chromatin was precleared with protein G agarose beads (Cell Signaling Technology, Waltham, MA, USA) for 2 h at 4 °C and immunoprecipitated with 8.5 μL of MEIS1-specific antibodies (Atlas Antibodies HPA056000), a negative control IgG antibody, or a positive control Histone H3 antibody (Cell Signaling Technology) overnight at 4 °C. .. PCRs were performed in triplicate using the HotStarTaq Master Mix (QIAGEN), and the primer sequences used are listed in .

    Article Title: Analysis of long-range interactions in primary human cells identifies cooperative CFTR regulatory elements
    Article Snippet: The Adaptive Focused AcousticsTM (AFA) Technology from Covaris was used in addition to enzymatic digestion with microccocal nuclease in order to produce DNA fragments of 150 to 900 bp. .. Chromatin was precleared with protein G magnetic beads (Cell Signaling Technology) for 2 h at 4°C and immunoprecipitation with the CTCF specific antibodies, a negative control IgG antibody or a positive control Histone H3 antibody (Cell Signaling Technology) were carried out overnight at 4°C. ..

    Article Title: Cx32 mediates norepinephrine-promoted EGFR-TKI resistance in a gap junction-independent manner in non-small-cell lung cancer.
    Article Snippet: Funding information National Natural Science Foundation of China, Grant/Award Number: 81473234; 81373439; Joint Fund of the National Natural Science Foundation of China, Grant/Award Number: U1303221; construction of technique plate for evaluation of the pharmacodynamics of new drugs in Xinjiang from the Department of Science and Technology of Guangdong province, Grant/Award Number: 2014A020209032; Department of Science and Technology of Guangdong Province, Grant/Award Number: 20160908; Fundamental Research Funds for the Central Universities, Grant/Award Number: 16ykjc01 Abstract The second‐generation EGFR‐TKI Afatinib is an irreversible ErbB family blocker used to treat patients with non‐small‐cell lung cancer (NSCLC).. Unfortunately, resistance to this drug develops over time, and patients are always under great psychological pressure.. A previous study showed that chronic stress hormones participate in EGFR‐TKI resistance via β2‐AR signaling via an IL‐6 dependent mechanism.

    Incubation:

    Article Title: Hepatocyte SLCO4C1 is a cAMP uptake transporter for inhibiting lipogenesis and a therapeutic target for MASLD.
    Article Snippet: Next, 1μl of Micrococcal Nuclease (CST, Boston, USA, Cat:10011) per IP sample was used to digest chromatin into varying lengths of fragments. .. Subsequently, the digested chromatin fragments were probed with 1μg anti-EGR1 antibody (CST, Boston, USA, Cat:4153S), positive control Histone H3 antibody (CST, Boston, USA, Cat:4620) or negative control Normal Rabbit IgG (CST, Boston, USA, Cat:2729) and incubated at 4°C for 12 h. The resulting immunoprecipitants containing specific DNA were subjected to PCR amplification using specific primers (Supplementary Table3). .. Slco4c1 overexpression by adeno-associated virus 8 (AAV8) The liver-specific AAV8 virions for the overexpression of Slco4c1-TBG-luciferase or controlTBG-luciferase were constructed and generated by BrainVTA (Wuhan, China).

    Article Title: Transcriptional co-factor Transducin beta-like (TBL) 1 acts as a checkpoint in pancreatic cancer malignancy
    Article Snippet: .. Antibodies were added as follows and incubated over night at 4°C: 1 μg negative control Normal Rabbit IgG (#2729, Cell Signaling), 10 μl positive control Histone H3 antibody (#4620, Cell Signaling), and 10 μl TBL1X-antibody (ab24548, Abcam). ..

    Polymerase Chain Reaction:

    Article Title: Hepatocyte SLCO4C1 is a cAMP uptake transporter for inhibiting lipogenesis and a therapeutic target for MASLD.
    Article Snippet: Next, 1μl of Micrococcal Nuclease (CST, Boston, USA, Cat:10011) per IP sample was used to digest chromatin into varying lengths of fragments. .. Subsequently, the digested chromatin fragments were probed with 1μg anti-EGR1 antibody (CST, Boston, USA, Cat:4153S), positive control Histone H3 antibody (CST, Boston, USA, Cat:4620) or negative control Normal Rabbit IgG (CST, Boston, USA, Cat:2729) and incubated at 4°C for 12 h. The resulting immunoprecipitants containing specific DNA were subjected to PCR amplification using specific primers (Supplementary Table3). .. Slco4c1 overexpression by adeno-associated virus 8 (AAV8) The liver-specific AAV8 virions for the overexpression of Slco4c1-TBG-luciferase or controlTBG-luciferase were constructed and generated by BrainVTA (Wuhan, China).

    Amplification:

    Article Title: Hepatocyte SLCO4C1 is a cAMP uptake transporter for inhibiting lipogenesis and a therapeutic target for MASLD.
    Article Snippet: Next, 1μl of Micrococcal Nuclease (CST, Boston, USA, Cat:10011) per IP sample was used to digest chromatin into varying lengths of fragments. .. Subsequently, the digested chromatin fragments were probed with 1μg anti-EGR1 antibody (CST, Boston, USA, Cat:4153S), positive control Histone H3 antibody (CST, Boston, USA, Cat:4620) or negative control Normal Rabbit IgG (CST, Boston, USA, Cat:2729) and incubated at 4°C for 12 h. The resulting immunoprecipitants containing specific DNA were subjected to PCR amplification using specific primers (Supplementary Table3). .. Slco4c1 overexpression by adeno-associated virus 8 (AAV8) The liver-specific AAV8 virions for the overexpression of Slco4c1-TBG-luciferase or controlTBG-luciferase were constructed and generated by BrainVTA (Wuhan, China).



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    Cell Signaling Technology Inc histone h3 positive control antibody
    ( A ) PET scans of MCL patient from case study pre (Left) and post (Right) 2 cycles of treatment with SCR. ( B ) Immunohistochemistry of diagnostic bone marrow biopsy showing infiltration of CD20 positive tumor cells (brown). ( C ) Immunohistochemistry of mediastinal lymph node biopsy at time of SCR resistant relapse showing infiltration with cells but lack of CD20 staining (brown). ( D ) Immunohistochemistry of cerebellum at autopsy showing CD20 positive (brown) cells. ( E ) qRT-PCR of CD20 ( MS4A1 ) expression in cells taken prior to treatment initiation and at time of resistant relapse. ( F ) ChIP assay of CD20 promoter pre (white) and 3 months post (black) treatment with SCR for total Histone 3 <t>(H3),</t> H3K9Me3, H3K27Me3, methylated cytosine (5MeC) and random rabbit antibody control. ( G ) Immunoblot for CD20 in cells cultured from patient’s 7 month relapse (353 cells) post 48 hours of in vitro cladribine treatment. Granta is positive control. 25*- 353 cells cultured in 25 nM cladribine for 3 months. β-actin is loading control. ( H ) qRT-PCR for CCND1, CCND2, CCND3, Sox11 and MS4A1 (CD20) levels in 353 (white), 48 hour 25 nM cladribine treated 353 (light grey), Granta 519 (dark grey), IB4 (black) and PBMCs (dotted). * p < 0.05 and compares 353 to cladribine treated 353. ( I ) FISH of 353 cells stained for DAPI (blue), IgH (green) and CCND1 (red). White arrows point to translocation events. Immunohistochemistry for CD20 (red) and cyclin D1(green) in ( J ) Granta 519 cells and ( K ) 353 cells. Scale bars are 10 μM.
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    Image Search Results


    ( A ) PET scans of MCL patient from case study pre (Left) and post (Right) 2 cycles of treatment with SCR. ( B ) Immunohistochemistry of diagnostic bone marrow biopsy showing infiltration of CD20 positive tumor cells (brown). ( C ) Immunohistochemistry of mediastinal lymph node biopsy at time of SCR resistant relapse showing infiltration with cells but lack of CD20 staining (brown). ( D ) Immunohistochemistry of cerebellum at autopsy showing CD20 positive (brown) cells. ( E ) qRT-PCR of CD20 ( MS4A1 ) expression in cells taken prior to treatment initiation and at time of resistant relapse. ( F ) ChIP assay of CD20 promoter pre (white) and 3 months post (black) treatment with SCR for total Histone 3 (H3), H3K9Me3, H3K27Me3, methylated cytosine (5MeC) and random rabbit antibody control. ( G ) Immunoblot for CD20 in cells cultured from patient’s 7 month relapse (353 cells) post 48 hours of in vitro cladribine treatment. Granta is positive control. 25*- 353 cells cultured in 25 nM cladribine for 3 months. β-actin is loading control. ( H ) qRT-PCR for CCND1, CCND2, CCND3, Sox11 and MS4A1 (CD20) levels in 353 (white), 48 hour 25 nM cladribine treated 353 (light grey), Granta 519 (dark grey), IB4 (black) and PBMCs (dotted). * p < 0.05 and compares 353 to cladribine treated 353. ( I ) FISH of 353 cells stained for DAPI (blue), IgH (green) and CCND1 (red). White arrows point to translocation events. Immunohistochemistry for CD20 (red) and cyclin D1(green) in ( J ) Granta 519 cells and ( K ) 353 cells. Scale bars are 10 μM.

    Journal: Oncotarget

    Article Title: Combined epigenetic and immunotherapy for blastic and classical mantle cell lymphoma

    doi: 10.18632/oncotarget.28258

    Figure Lengend Snippet: ( A ) PET scans of MCL patient from case study pre (Left) and post (Right) 2 cycles of treatment with SCR. ( B ) Immunohistochemistry of diagnostic bone marrow biopsy showing infiltration of CD20 positive tumor cells (brown). ( C ) Immunohistochemistry of mediastinal lymph node biopsy at time of SCR resistant relapse showing infiltration with cells but lack of CD20 staining (brown). ( D ) Immunohistochemistry of cerebellum at autopsy showing CD20 positive (brown) cells. ( E ) qRT-PCR of CD20 ( MS4A1 ) expression in cells taken prior to treatment initiation and at time of resistant relapse. ( F ) ChIP assay of CD20 promoter pre (white) and 3 months post (black) treatment with SCR for total Histone 3 (H3), H3K9Me3, H3K27Me3, methylated cytosine (5MeC) and random rabbit antibody control. ( G ) Immunoblot for CD20 in cells cultured from patient’s 7 month relapse (353 cells) post 48 hours of in vitro cladribine treatment. Granta is positive control. 25*- 353 cells cultured in 25 nM cladribine for 3 months. β-actin is loading control. ( H ) qRT-PCR for CCND1, CCND2, CCND3, Sox11 and MS4A1 (CD20) levels in 353 (white), 48 hour 25 nM cladribine treated 353 (light grey), Granta 519 (dark grey), IB4 (black) and PBMCs (dotted). * p < 0.05 and compares 353 to cladribine treated 353. ( I ) FISH of 353 cells stained for DAPI (blue), IgH (green) and CCND1 (red). White arrows point to translocation events. Immunohistochemistry for CD20 (red) and cyclin D1(green) in ( J ) Granta 519 cells and ( K ) 353 cells. Scale bars are 10 μM.

    Article Snippet: Anti-Cyclin D1 antibody [SP4] (Immunoblot) (abcam, ab16663), Anti-β Tubulin Antibody (H-235) (Santa Cruz sc-9104), Anti-H3K27Me3 (Millipore 05-1951), Anti-H3K9Me3 (Millipore 07-523), Anti-H3K27Me2 D18C8 (Cell Signaling #9728), Anti-H3k9Me2 (Millipore 07-521), Anti-β-actin (Millipore MAB1501), Anti-DUSP2 C-20 (Santa Cruz sc-1620-R), Anti-Caspase 3 (Cell Signaling #9662), Anti-Caspase 9 (Cell Signaling #9501), Anti-Cyclin D1 (IHC) (Santa Cruz sc-8392), Anti-CD20 (Santa Cruz sc-19990), normal rabbit antibody (Santa Cruz Biotechnology sc-2345), histone H3 positive control antibody (Cell Signaling #9715).

    Techniques: Immunohistochemistry, Diagnostic Assay, Staining, Quantitative RT-PCR, Expressing, Methylation, Control, Western Blot, Cell Culture, In Vitro, Positive Control, Translocation Assay